Microfab~cate~ Cyclical Electrical Field Flow’ Fractionation
نویسندگان
چکیده
A cyclical electrical field flow fractionation (CEFFF) microsystem is demonstrated for the first time in this paper. Retention of polystyrene nanoparticles is accomplished and the effect of varying parameters such frequency is explored. This new sub-micron size particle separation technique can be used to separate cellular components, viruses and bacteria, proteins and DNA, colloids and emulsions; and thus, can be used as a lab-on-a-chip sample preparation method. Due to handling of smaller sample volumes in the system, the separation is faster than in comparable macrosystem.
منابع مشابه
Characterization of polymerized liposomes using a combination of dc and cyclical electrical field-flow fractionation.
Characterization of polymerized liposomes (PolyPIPosomes) was carried out using a combination of normal dc electrical field-flow fractionation and cyclical electrical field-flow fractionation (CyElFFF) as an analytical technique. The constant nature of the carrier fluid and channel configuration for this technique eliminates many variables associated with multidimensional analysis. CyElFFF uses...
متن کاملParticle Based Modeling of Electrical Field Flow Fractionation Systems
Electrical Field Flow Fractionation (ElFFF) is a sub method in the field flow fractionation (FFF) family that relies on an applied voltage on the channel walls to effect a separation. ElFFF has fallen behind some of the other FFF methods because of the optimization complexity of its experimental parameters. To enable better optimization, a particle based model of the ElFFF systems has been deve...
متن کاملMiniaturized flow fractionation device assisted by a pulsed electric field for nanoparticle separation.
Electric field flow fractionation (EFFF) is a powerful separation technique based on an electrical field perpendicular to a pressure-driven flow. Previous studies of microelectric field flow fractionation (micro-EFFF) indicate that separation performance was limited due to a weak effective electric field caused by polarization layers on the electrode surfaces. In this work, we report on a micro...
متن کاملMechanistic investigation of nanoparticle motion in pulsed voltage miniaturized electrical field flow fractionation device by in situ fluorescence imaging.
In our previous study, we reported a miniaturized electrical field flow fractionation device (micro-EFFF) that used a pulsed voltage (PV) to increase the effective electric field and, hence, improved the separation performance. In this work, we developed two micro-EFFFs with planar or segmented electrode design and investigated the particle movement in the flow channels under a PV. Numerical si...
متن کامل